DNMT1 Pure 18/DNMT1 50ug

DNA methylation of the 5' position of the cytosine ring within CpG dinucleotides is essential for embryonic development. This methylation

may affect transcriptional repression, formation of compact chromatin structures, X chromosome inactivation, and imprinting control. Three

important DNA (Cytosine-5) methyltransferases (DNMTs) include DNMT1, DNMT3A, and DNMT3B. DNMT1 contains an N-terminal

regulatory domain that may target it to replication foci, and a C-terminal catalytic domain that is homologous to bacterial cystosine

5-methylases. DNMT1 knockout mice have reduced DNA methylation, defects in imprinting, and derepression of endogenous retroviruses,

and are embryonic lethal. DNMT1 is responsible for DNA methylations that occur after replication, while DNMT3A and DNMT3B act to

establish new methylation patterns during embryogenesis. DNMT1 and DNMT3A are expressed in most fetal tissues, while DNMT3B is

expressed only in fetal liver. All of these DNMTs are found in various adult tissues and show increased expression in some tumors and cancer

cell lines. Thus, DNMT methylation of CpG dinucleotides is important for regulating DNA structure during development.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.