ERK1/2 (pT202/pY204) PE 20A 50Tst

The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events

at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the

proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases,

ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as

growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and

eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation

of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other

cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the

active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the

cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers.

The 20A monoclonal antibody recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and

pT184/pY186 of human ERK2. The orthologous phosphorylation sites in murine ERK1 and ERK2 are T203/Y205 and T183/Y185.

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.