CIP4 Pure 21/CIP4 150ug

Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle

between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human

platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae.

Similarly, CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis.

CDC42-interacting protein 4 (CIP4) was identified in a yeast-two hybrid screen for proteins that bind CDC42. Another variant of CIP4,

CIP4/2, was identified that contains an extra 56 amino acids and has 71% identity with CIP4 (or CIP4/1). CIP4 contains a C-terminal SH3

domain and an N-terminal domain that is homologous to non-catalyitic motifs in the tyrosine kinase Fer. The mRNA expression of CIP4 is

highest in skeletal muscle, heart, and placenta. Overexpression of CIP4 in Swiss 3T3 cells reduces the amount of stress fibers and leads to

clustering of CIP4 to foci at the dorsal side of the cells. In addition, CIP4 binds the Rho-GTPase activating protein RICH and the cytoskeletal

protein WASP. Coexpression of CIP4 and WASP in Cos-7 cells leads to WASP association with microtubules. Thus, CIP4 is involved in

various protein-protein interactions associated with cytoskeletal dynamics.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.