Prostasin Pure 2/Prostasin 150ug

Prostasin is a serine protease expressed as both GPI-anchored and secreted forms. Prostasin displays trypsin-like enzymatic activity by

hydrolyzing substrates, such as D-Pro-Phe-Arg-AMC. This activity is inhibited by aprotinin, antipain, leupeptin, and benzamidine. The

highest levels of prostasin protein are found in semen and the prostate, but many other tissues express prostasin at low levels. The sequence of

prostasin is similar to other proteases, such as prostase, testisin, plasma kallikrein, and acrosin. In seminal fluid, prostasin can be found in

complex with prostasin-binding protein, which can inhibit prostasin enzymatic activity. Prostasin mRNA is found in normal prostate epithelial

cells and non-invasive prostate cancer cells, but is not expressed in invasive prostate cancer cell lines. Expression of prostasin in the invasive

prostate cancer cell lines, DU-145 and PC-3, reduces the invasiveness of the cells in vitro. Thus, prostasin is a serine protease that may have

roles in normal prostate function and in suppression of tumor cell invasion.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.