GBF1 Pure 25/GBF1 50ug

The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and

regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance

Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the

C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is

ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal

heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various

organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat

protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide

exchange factor that activates ARFs through a mechanism that interferes with BFA action.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.