FHIT Pure 30/FHIT 50ug

Multiple tumor-derived cell lines have homozygous deletions in a 200-300 kb region in chromsome 3p14.2. This region includes the fragile

site locus FRA3B, which may contain deletions in tumor suppressor genes. Abnormalities in the expression and structure of a gene, FHIT, that

spans the FRA3B locus have been observed in a variety of carcinomas. FHIT (Fragile Histidine Triad) is widely expressed in normal

embryonic and adult tissues and is absent in lung, renal, and gastric tumors. Exposure of FHIT heterozygous knock-out mice to carcinogens

produces increased tumorigenicity similar to that observed in Muir-Torre familial cancer syndrome. The only known function of FHIT is as a

diadenosine 5',5'''-P1, P3-triphosphate (Ap3A) hydrolase. Both in vitro and in vivo, FHIT regulates the level of Ap3A and adenosine (5')

tetraphosphate(5') nucleoside. However, this hydrolase activity may not be involved in FHIT tumor suppressor activity, since a mutant FHIT

that binds but does not hydrolyze Ap3A is as effective as the wild-type in reducing tumorigenicity in mouse cancer models. Thus, FHIT

activity may be essential for a unique antiproliferative signaling pathway.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.