Drp1 Pure 22/Drp1 50ug

In culture, cell density can have profound effects on gene expression, enzymatic activity, and cell signaling pathways. Using differential

screening of cDNAs from low-passage nontumorigenic teratocarcinoma cells versus high passage tumorigenic cells, a protein was identified

that is regulated in cell-density dependent manner. This protein, density-regulated protein 1 (drp1), contains putative sites for N-myristoylation

and phosphorylation sites for cAMP and/or cGMP-dependent kinase, casein kinase II, and PKC. The expression of drp1 is enriched in high

density cultures of both nontumorigenic and tumorigenic cell lines and is widely detected in adult organs, especially skeletal and cardiac

muscle. In addition, increased expression of drp1 is not due to growth arrest as a result of serum starvation or TGF-β treatment nor is it a result

of factors found in the media of high density cultures. Interestingly, drp1 is expressed highest in skeletal and cardiac muscle where unique

cell-cell contacts are involved in muscle cell membrane depolarization and contraction. Thus, drp1 expression may be regulated by signaling

pathways related to specific types of cell-cell contacts.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.