Central nervous system plasticity is essential to the processes of learning and memory. Plasticity involves changes in synaptic transmission
efficiency and results from the activation of excitatory glutamate receptors. Analysis of changes in gene expression during kainate-induced
hippocampal plasticity led to the identification of a Candidate Plasticity-related Gene (CPG16). CPG16 protein is a Ser/Thr protein kinase that
is also closely related to the Ca2+/calmodulin- dependent protein kinases (CaM kinases). CPG16 is 98% identical to the C-terminal region of
the product of the KIAA0369 gene, a 96 kDa protein whose N-terminal region strongly resembles the neural protein, doublecortin. In resting
cells, Cam Kinase VI (CPG16) is found in the cytoplasm and has relatively low level of phosphorylation activity. However, treatment of cells
with cAMP-elevating agents (forskolin, 8-bromo-cAMP) significantly enhances this phosphorylation activity and induces a partial shift of
Cam Kinase VI (CPG16) into the nucleus. In addition, overexpression of Cam Kinase VI (CPG16) inhibits cAMP-stimulated CREB activity.
Thus, Cam Kinase VI (CPG16) is Ser/Thr protein kinase that is thought to be a downstream negative regulator of cAMP/PKA-induced
transcription.
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注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.