Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately attached to mitotic spindles before the
onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until
proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached
kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity.
Bub3 contains four WD repeats, three in the N-terminus and one in the C-terminus, and a central Bub1-binding domain. During prophase and
prometaphase, Bub3 localizes to the kinetochore before attachment to microtubules. In addition, taxol-induced formation of lagging
chromosomes due to a delay of cell cycle progression increases the level of Bub3 co-localized with kinetochores, while correctly aligned
chromosomes found in metaphase do not exhibit this co-localization. Thus, Bub3, in association with Bub1, may be important for sensing
kinetochore attachment to microtubules during the prometaphase to metaphase transition.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
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