Mcm proteins are nuclear proteins that perform essential functions in the regulation of chromatin replication. They are primarily bound to
chromatin during G1 and the beginning of S-phase, but are found in the nucleosol in postreplicative cells. Release of the bound Mcm proteins
may be a mechanism for prevention of chromatin re-replication during S-phase. MCM6 is homologous to the yeast replication protein Mis5
and contains a DEFD-box motif and nucleotide-binding domain in the central region that are conserved in Mcm proteins. MCM6 forms a
complex with MCM2, -4, and -7, which binds histone H3. In addition, the subcomplex of MCM4, -6, and -7 has helicase activity, which is
mediated by the ATP-binding activity of MCM6 and the DNA-binding activity of MCM4. In Xenopus, formation of pre-replication complexes
that include Mcm protein complexes requires phosphorylation, since intermediate phosphorylation of MCM4 correlates with Mcm protein
complex formation and chromatin binding. Thus, Mcm proteins form heterocomplexes that participate in many different functions required for
chromatin replication, such as DNA binding, helicase activity, and chromatin structure rearrangement.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
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