Myosin Vb/Myr 6 Pure 18/Myr 6 50ug

Myosins are proteins that form oligomers consisting of two heavy chains and varying numbers of light chains. Many classes of myosin

superfamily proteins which have similar N-terminal motor domains but divergent C-terminal domains have been characterized. Class V

myosins interact via their C-terminal tails with specific proteins involved in organelle transport. In vertebrates, the myosin V family includes

mouse myosin Va (dilute myosin) and Vb, rat myosin Vb (myosin from rat 6; myr 6), and chicken p190. Myr 6 includes an N-terminal

globular domain, six IQ motifs in the neck region, two coiled-coil domains on each side (cc) of a PEST calpain cleavage region, and a

C-terminal tail that is homologous to dilute myosin, AF-6, and GAD. Myr 6 is expressed highly in mouse heart, kidney, testis, liver, and lung.

In rat brain, it is expressed highly in dentate gyrus,medial mamillary nuclei, amygdala, and choroid plexus. The tail region of myr 6 interacts

with the C-terminal domain of BERP, a RING-B-box-coiled-coil protein that has been implicated in PC12 cell neurite outgrowth. Thus, myr 6

may function in the transport of organelles in a variety of neural and non-neuronal tissues.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.