Intercellular communication in an organism requires a correct spatial assembly to maximize the transfer of signals between cells. The type of junctions between cells determine the functional characteristics of a tissue. Zonula adherens, or adherens junctions, are a specialized form of cell-to-cell contact designed to mechanically attach the cells, through their actin cytoskeleton, to one another or to the extracellular matrix. The transmembrane linker proteins that bring the two opposing cell membranes together are the cadherins. They interact with the cytoskeleton through their associated cytoplasmic catenin proteins. In addition, cadherins are critical regulators of epithelial junction formation. Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. β-catenin is a 92 kDa protein that binds to the cytoplasmic tail of cadherin. Although the α- and β-catenins have been cloned, very little is known about their biochemical roles. However, a link between β-catenin and colon cancer has been described. β-catenin co-immunoprecipitates with the APC tumor suppressor protein in human colorectal tumor cell lines. The membrane associated protein pp120 (p120cas) was identified as a Tyr kinase substrate that is phosphorylated in src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila armadillo protein and the β-and γ-catenin proteins. This is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats. In the cell, pp120 is localized to the cadherin/catenin cell adhesion complex. Thus, zonula adherens are critical intercellular contact sites that involve the interaction of numerous cell adhesion molecules.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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