hRad9 Pure 56/hRad9 50ug

Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely

replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription.

In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found

in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as

hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and

gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage

induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as

apoptosis, in response to DNA damage.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.