Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3'-splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), and Prp18p (hPrp18). Prp16 is a DExD/DExH-box family RNA dependent ATPase, which can unwind RNA duplexes. hPrp16 is 41% identical to its yeast counterpart and chimeric yeast-human Prp16 can rescue a yeast Prp16 knockout strain. The non-conserved N-terminal region of hPrp16 is essential for viability, required for nuclear localization, and capable of binding to the splicesome during the second catalytic step. Thus, hPrp16 is a non-snRNP that is critical for spliceosomal function in the process of pre-mRNA splicing.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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