p230 Trans Golgi Pure 15 50ug

The Golgi apparatus is a very complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and

regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. p230 trans Golgi is a peripheral membrane

protein associated with the cytosolic face of the trans Golgi network (TGN). Protein sequence analysis indicates that it is highly hydrophilic

and contains two proline-rich regions, as well as a high frequency of heptad repeats which are characteristic of α-helices that form dimeric

coiled-coiled structures. Additionally, p230 trans Golgi contains a ESLALEELEL sequence, a motif of the acidic granin proteins that are

found in secretory granules of neuroendocrine cells. p230 trans Golgi cycles between the cytosol and the TGN via non-clathrin coated vesicles

in a G protein-dependent manner. The localization of p230 trans Golgi to the TGN is dependent on aromatic residues in its C-terminal

non-coiled coil domain. Thus, p230 trans-Golgi is aTGN-specific protein that is important for the biogenesis of distinct populations of

non-clathrin coated vesicles and, thus, functions in vesicular transport from the TGN.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.