p54[nrb] Pure 3/p54nrb 150ug

Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes that contain multiple snRNPs and a number of

non-snRNP splicing factors, such as hPRP17 and hPRP18. p54 [nrb] (nuclear RNA-binding protein, 54 kDa) is a non-snRNP RNA splicing

factor. It binds and enhances the activity of topoisomerase I, the enzyme that nicks DNA to relieve torsional stress during replication and

transcription. p54 [nrb] is the the human homolog of mouse NonO and bovine IPEB. The N-terminal half of p54 [nrb] contains two RNA

recognition motifs (RRMs), that allow it to bind single stranded RNA. These motifs are included in a region that is 71% homologous with the

human splicing factor PSF and 42% homologous with the Drosophila puff-specific protein BJ6. In addition to its role in RNA splicing, p54

[nrb] functions as a DNA transcription factor in multiple cell types. The N-terminal portion of p54 [nrb] also mediates its interaction with

DNA. The ability of p54 [nrb] to bind DNA, but not RNA, is thought to require post-translational modification. Thus, p54 [nrb] can function

as either an RNA splicing factor or a DNA transcription factor in a post-translational-dependent manner.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.

3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.