Damaged or erroneous DNA may result from the effects of environmental factors or synthesis errors committed by DNA polymerases during replication. If unchecked, these errors might accumulate genetic damage such that the cell could no longer properly function. Thus, DNA repair processes involve mechanisms for the excision of damaged sequences and the resynthesis and ligation of the proper sequence. DNA polymerase ε functions in DNA replication and repair. It consists of a large catalytic subunit, reportedly ranging in size from 220 kD to 261 kD, and a 55 kDa small subunit that is thought to function as an adaptor between the catalytic subunit and accessory proteins such as DPB2, DPB3, and proliferating cell nuclear antigen (PCNA). The polymerase and proofreading exonuclease activities are located in the N-terminal portion of the catalytic subunit. Although the C-terminal portion contains a putative Zn2+ finger DNA-binding domain, the function of this region is not required for enzyme activity. The interaction of DNA Pol ε with PCNA promotes primer recognition and DNA synthesis. Thus, DNA Pol ε is essential for DNA replication and the maintenance of accurate DNA sequence, which ensures cellular function and viability.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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