Telomeres, the physical ends of eukaryotic chromosomes, consist of tracts of TTAGGG repeats that end in 3' single strand protrusions called G-strand overhangs. TRF2 (TTAGGG Repeat binding Factor-2) and TRF1 are two proteins that bind the TTAGGG repeats of mammalian telomeres. Both proteins are ubiquitously expressed and exhibit a similar overall structure with a C-terminal DNA binding motif related to the protooncogene Myb and an N-terminal putative dimerization domain. While TRF1 is a negative regulator of telomere length maintenance, TRF2 is involved in the protection of chromosome ends from end-to-end fusion. Telomere integrity is maintained through the formation of "t loops" which are made by the insertion of the G-strand overhang into the duplex repeat region. This structural alteration is mediated by TRF2 and is the primary mechanism for the sequestration and protection of telomeres from DNA damage responses. Cells that lack functional TRF2 undergo loss of G-strand overhangs, innapropriate DNA repair resulting in end-to-end chromosome fusion, cell cycle arrest and apoptosis. Thus, TRF2 is a unique and vital component for the maintenance of chromosome structure and function.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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