Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein kinase [PAK]) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of the second class lack GTPase binding sites, but are similar to the former class throughout the catalytic domain. The latter class includes GC kinase, HPK, KHS, KRS1 & 2, MST1, 2, & 3, and SOK-1. MST3 (Mammalian Sterile Twenty-like) is a ubiquitous cytoplasmic kinase that contains an N-terminal kinase domain and a C-terminal regulatory domain. It shares extensive sequence similarity with SOK-1 and is similarly autophosphorylated and activated. MST3 remains distinct in that it prefers Mn2+ to Mg2+ as a divalent cation and uses GTP and ATP as phosphate donors. It does not respond to a variety of mitogenic and stress stimuli and fails to activate known mammalian MAPK pathways. Although its function is undefined, MST3 may function in a novel intracellular signaling pathway.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
