Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs). As a prerequisite to initiation, the initiator methionyl-tRNA (Met-tRNAf), in a ternary complex with eIF-2 and GTP, binds to the 40S ribosomal subunit. This 40S preinitiation complex is positioned at the initiation AUG codon to form the 40S initiation complex. This complex is joined by the 60S ribosomal subunit to form the 80S initiation complex, which functions as a peptidyl transferase. The joining of the 60S subunit to the 40S initiation complex requires eIF-5. This protein associates with the 40S initiation complex, specifically with the β subunit of eIF-2, prior to binding of the 60S subunit. eIF-5 induces GTP hydrolysis of the ternary complex bound to the 40S ribosome. This results in the release of eIF-2 and GDP from the 40S subunit and allows for interaction with the 60S subunit. Furthermore, eIF-5 is thought to be involved in recognition of the start codon and interacts with eIF-3, an additional factor required for 40S initiation complex formation. Thus, eIF-5 is essential to the assembly and proper function of the eukaryotic machinery. eIF-5 has a calculated molecular weight of 49 kDa, but has been reported to migrate in a range between 49-53 kDa.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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