5-Lipoxygenase Pure 33 50ug

5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, which are important inflammatory and

vasoconstrictive metabolites. It is activated in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene,

abundantly expressed in placenta, lung, and leukocytes, encodes a protein of 674 amino acids with an apparent molecular weight of 78kDa.

5-LO is a Ca2+ and ATP-dependent enzyme that translocates from the cytosol to either a nuclear or plasma membrane compartment following

activation. A proline-rich domain of 5-LO (amino acids 566-577) has been identified as a binding site for the PTyr-binding protein, Grb2. This

Grb2 site links tyrosine kinases with activation and redistribution of 5-LO. Furthermore, tyrosine kinase inhibitors increase the activity of

5-LO and block the enzyme's subcellular redistribution.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.