E-Cadherin Pure 36/E-Cadherin 50ug

• 产品编号：BD-610181      品牌：BD-Pharmingen       原厂货号：610181
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

E-钙粘蛋白是一个120-kDa的定位于上皮细胞的粘着部位的跨膜糖蛋白，它通过细胞质的链蛋白与细胞骨架相互作用。除了是钙依赖性的粘附分子，E-钙粘蛋白也是上皮粘结形成的一个关键调节因子，其与链蛋白的关联作用在细胞间粘附过程中是必要的。这些E-钙粘蛋白/链蛋白的复合物与皮质肌动蛋白一起束缚于粘着小带和侧粘着斑。酪氨酸磷酸化可以破坏这些复合物，从而导致细胞粘附性的改变。在高度侵袭性​​、低分化性的肿瘤中，E-钙粘蛋白的表达往往是下调的。E-钙粘蛋白在这些细胞中的表达上调可减少肿瘤侵袭性。因此，E-钙粘蛋白的表达的缺失或功能的缺失似乎是肿瘤的发展的一个重要步骤。在磷酸化状态下，36/E-钙粘蛋白单克隆抗体也可识别E-钙粘蛋白的胞质结构域。本抗体的多肽免疫原来自人E-钙粘蛋白的735-883位氨基酸。

原厂资料：

E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. The 36/E-Cadherin monoclonal antibody recognizes the cytoplasmic domain of E-Cadherin, regardless of phosphorylation status. The peptide immunogen was generated from human E-Cadherin aa. 735-883.

注意事项：

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Store undiluted at -20°C.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography