STAT1 (pY701) PE-CF594 4A 50Tst

产品编号：BD-562674 品牌：BD-Pharmingen 原厂货号：562674
产品分类：抗体 > 一抗 > 磷酸化抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including
interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK
family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the
nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed
and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated
Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface
receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids. In
response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become
tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that
includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes
(ISGs).
The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1α and Stat1β.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.