Ms CD11B PE-CF594 M1/70 25ug

• 产品编号：BD-562317      品牌：BD-Pharmingen       原厂货号：562317
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

原厂资料：

The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170-kDa type 1
transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1
integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2
(CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic
cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the
same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi
binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red (eg 610/20-nm filter).

注意事项：

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.

3.An isotype control should be used at the same concentration as the antibody of interest.

4.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
6.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.