OCT3/4A PE O50-808 50Tst

• 产品编号：BD-561556      品牌：BD-Pharmingen       原厂货号：561556
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Development of a multicellular organism from a single fertilized egg is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a
member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem (ES) cell lines and embryonal carcinomas
(EC). The human POU5F1 gene can encode various splice variants, two of which are Oct3/4A and Oct3/4B. Both isoforms share identical POU
DNA-binding and C-terminal domains but differ in their N-terminal domain. The N-terminal domain of Oct3/4B is inhibitory to the DNA binding
domain and therefore cannot stimulate transcription of Oct3/4-dependent genes. Oct3/4B can be detected in both pluripotent and some differentiated
cell types in both the nucleus and cytoplasm, but its function is unclear. There is not an equivalent to Oct3/4B in mouse. Oct3/4A is expressed in the
nucleus and has been demonstrated to orchestrate the transcription of Oct3/4-dependent genes. It has been demonstrated that the expression of Oct3/4
isoforms can vary greatly in different cell types, and discrimination of these is crucial for assessing Oct3/4 expression and function. The O50-808
monoclonal antibody recognizes human Oct3/4 Isoform A and mouse Oct3/4.
 

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.