描述:
The BD Horizon™ V450 Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such
as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five BD
Horizon™ V450-conjugated antibodies is designed to label lineage marker-positive cells for exclusion to facilitate the flow cytometric
identification of lineage marker-negative hematopoietic progenitor cells in mouse bone marrow. Components include clone 500A2, which
recognizes Mouse CD3e; M1/70, which recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76,
mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. The BD Horizon™ V450 Mouse Lineage Isotype Control Cocktail
contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be
combined with the BD Horizon™ V450 Mouse Lineage Antibody Cocktail, and the BD Horizon™ V450 Mouse Lineage Isotype Control
Cocktail, to further characterize hematopoietic progenitor subpopulations.
The antibodies are conjugated to BD Horizon™ V450 which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with
BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.