Hu CD163 PE GHI/61 25Tst

• 产品编号：BD-560933      品牌：BD-Pharmingen       原厂货号：560933
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The GHI/61 monoclonal antibody specifically binds to human CD163. CD163 is also known as Scavenger receptor cysteine-rich type 1
protein M130 (M130), Hemoglobin scavenger receptor and Macrophage-associated antigen. CD163 is a 110-130 kDa transmembrane
glycoprotein. CD163 is a monocyte/macrophage-restricted antigen expressed on the majority of tissue macrophages and peripheral blood
monocytes. CD163 belongs to the scavenger receptor superfamily. Its expression on monocytes is upregulated upon cellular activation. CD163
expression reportedly changes on monocytes and macrophages as these cells differentiate. This finding suggests a role for this molecule in the
differentiation and/or regulation of monocyte and macrophage function. CD163 may play a role in the clearance and endocytosis of
hemoglobin and haptoglobin complexes by macrophages.
It has been reported (Maniecki et al., 2011) that the presence of calcium impacts the binding affinity of clone GHI/61 to CD163. There is a
variation in detecting CD163 positive monocytes when the cells are prepared with different anticoagulants, where heparin was observed to
have the highest inhibitory effect on clone GHI/61.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.