Hu CD4 Horizon V500 RPA-T4 100Tst

产品编号：BD-560768 品牌：BD-Pharmingen 原厂货号：560768
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

100Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human immunodeficiency virus (HIV)]
present on T-helper/inducer cell populations. This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and
reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral
blood monocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.
The antibody is conjugated to BD Horizon. V500, which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415 nm and Em Max at 500 nm. BD Horizon
V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD
Horizon V500, visit bdbiosciences.com/colors.
When compensating dyes in this spectral range (such as Horizon. V500 and AmCyan), the most accurate compensation can be obtained
using single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in
spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values
for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover
therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.