Hu Regulatory T Cell Sorting Kit

• 产品编号：BD-560753      品牌：BD-Pharmingen       原厂货号：560753
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

Human Regulatory T Cell Sorting Kit
The immune system protects individuals from a broad range of pathogenic microorganisms while avoiding inappropriate or extreme immune
responses, such as autoimmune or allergic immune responses that could be harmful. Adaptive immunity is largely mediated by T and B
lymphocyte populations that can specifically recognize a diverse constellation of foreign antigens. Through processes of rapid clonal
expansion and differentiation, antigen-stimulated lymphocytes generate a vast array of potent effector and memory cell progeny to successfully
rid the body of the offending foreign antigen.
A distinct class of T cells, called regulatory T cells (Treg), safeguards against the immune system's responsiveness to self antigens and
restrains excessive responsiveness to foreign antigens that could be harmful to the host. Natural CD4+ T regulatory cells (nTreg) are
generated in the thymus as a functionally mature subpopulation of T cells. These cells emigrate to the peripheral T cell pool and constitutively
express medium to high levels of CD25 (IL-2 receptor α chain) and Foxp3 (forkhead box P3). The transcription factor Foxp3 is a member of
the forkhead/winged-helix family of transcription factors and is a master regulator of Treg development and function. CD127 (IL-7 receptor α
chain) constitutes another useful cell surface marker for identifying Treg cells. CD127 is downregulated on Treg cells, inversely correlating
with the expression of Foxp3. Inducible CD4+ T regulatory cells (iTreg) can arise from mature peripheral naïve CD4+ T cells not expressing
CD25 and Foxp3 (CD4+CD25-Foxp3- cells).
Recent studies indicate that natural human CD4+FoxP3+ T cells can be subdivided into phenotypically- and functionally-distinct subsets
based on the coexpressed levels of Foxp3, CD25 and CD45RA. The CD45RA marker is particularly useful for identifying resting CD4+
natural Treg cells (CD45RA+) as well as in vivo-activated CD4+ Treg (CD45RA-) when used with Foxp3, CD25, and CD127 markers.
Phenotypic and functional studies using these markers provide a basis for high resolution analyses of the dynamics of Treg cell generation and
differentiation in development, age and disease states.
The BD™ Human Regulatory T Cell Sorting Kit provides reagents that allow investigators to stain viable human peripheral blood
mononuclear cells (PBMC) or an enriched CD4+ T cell fraction of PBMC and to purify CD4+CD25+CD127lo cells (optional CD45RA+).
These cells can then be tested functionally or used as precursors for expansion and differentiation cultures to generate activated Treg cells.
Other cellular fractions such as CD4+CD25-CD127+ cells (naïve CD4+ T cells) can simultaneously be purified by flow cytometric sorting for
subsequent functional characterization. In addition, fluorescent anti-human CD45RA antibody is included to facilitate further characterization
or sorting of naïve (CD45RA+) or activated/memory (CD45RA-) CD4+Foxp3+ or CD4+Foxp3- T lymphocytes.

原厂资料：

注意事项：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.