PARP Cleaved Form Alexa700 F21-852 50Tst

产品编号：BD-560640 品牌：BD-Pharmingen 原厂货号：560640
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

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描述：

PARP (Poly [ADP-Ribose] Polymerase) is a 113-kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units
from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased
in cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage.
Additionally, PARP is a target of the caspase protease activity associated with apoptosis. The PARP protein consists of an N-terminal
DNA-binding domain (DBD) and a C-terminal catalytic domain separated by a central automodification domain. During apoptosis, Caspase-3
cleaves PARP at a recognition site (Asp Glu Val Asp Gly) in the DBD to form 24- and 89-kDa fragments. This process separates the DBD
(which is mostly in the 24-kDa fragment) from the catalytic domain (in the 89-kDa fragment) of the enzyme, resulting in the loss of normal
PARP function. It has been proposed that inactivation of PARP directs DNA-damaged cells to undergo apoptosis rather than necrotic
degradation, and the presence of the 89-kDa PARP cleavage fraction is considered to be a marker of apoptosis.
A peptide corresponding to the N-terminus of the cleavage site (Asp 214) of human PARP was used as the immunogen. The F21-852
monoclonal antibody reacts only with the 89-kDa fragment of human PARP-1 that is downstream of the Caspase-3 cleavage site (Asp214) and
contains the automodification and catalytic domains. It does not react with intact human PARP-1. Cross-reactivity with other members of the
PARP superfamily is unknown. It may also recognize cleaved PARP in a number of other species due to the conserved nature of the
molecule, although this has not been tested at BD Biosciences Pharmingen.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.