p53 (pS37) Alexa 647 J159-641.79 50Tst

产品编号：BD-560280 品牌：BD-Pharmingen 原厂货号：560280
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The p53 protein is critical to regulation of normal cell growth and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number
of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations
and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53
suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a
sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream
of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle
creatine kinase, which primarily prevent cell proliferation. MDM2 mediates feedback inhibition of p53, which is prevented by
phosphorylations of p53 amino-terminal serines and threonines. Upon exposure to DNA damage-inducing agents, ATR and DNA-PK (ataxia
telangiectasia- and Rad3-related and DNA-dependent protein kinases, respectively) phosphorylate p53 at serine 37 (S37). This
phosphorylation disrupts the binding of MDM2 to p53, allowing the cell’s stress responses to proceed.
The J159-641.79 monoclonal antibody recognizes p53 phosphorylated at S37.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results..
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.