Stat1 (pS727) Alexa 647 K51-856 50Tst

产品编号：BD-560190 品牌：BD-Pharmingen 原厂货号：560190
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins,
interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and
subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote
transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a
cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex,
which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively
spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids. In response to the binding of IFNα, IFNγ,
EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated
to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for
modulating the transcription of the interferon-stimulated genes (ISGs). Furthermore, phosphorylation of serine 727 (S727) of Stat1 may occur in
response to pathogens, cytokines, UV irradiation, and engagement of T and B cell antigen receptors. This second phosphorylation event is
necessary for fully effective activation of transcription and may differentially regulate gene activation by interacting with other regulatory factors,
such as p53 in the apoptotic pathway.
The K51-856 monoclonal antibody recognizes the phosphorylated S727 in the C-terminal transactivating domain of human Stat1α; this site is not
present in Stat1β.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.