Stat1 (pS727) PE K51-856 50Tst

• 产品编号：BD-560069      品牌：BD-Pharmingen       原厂货号：560069
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including
interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated
JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to
the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially
expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3
(Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a
specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional
C-terminal amino acids. In response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits
become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active
complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the
interferon-stimulated genes (ISGs). Furthermore, phosphorylation of serine 727 (S727) of Stat1 may occur in response to pathogens,
cytokines, UV irradiation, and engagement of T and B cell antigen receptors. This second phosphorylation event is necessary for fully
effective activation of transcription and may differentially regulate gene activation by interacting with other regulatory factors, such as p53
in the apoptotic pathway.
The K51-856 monoclonal antibody recognizes the phosphorylated S727 in the C-terminal transactivating domain of human Stat1α; this
site is not present in Stat1β.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.