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Phospho ERK1/2 CBA Flex Set 100Tst

 
包装: 100Tst
运保温度: stored at 4 °C
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标准价: ¥客户可见
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1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

其它组分:

Phospho ERK1/2 (T202/Y204) PE Detection Reagent【子货号:51-9003896】
Phospho ERK1/2 (T202/Y204) Standard【子货号:51-9004815】
Phospho ERK1/2 (T202/Y204) Capture Bead C4【子货号:51-9005192】


描述:

The BD™ CBA Phospho ERK1/2 (T202/Y204) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat extracellular
signal regulated kinases 1/2 (ERK1/2), a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been
threonine-phosphorylated at Thr-202 and tyrosine-phosphorylated at Tyr-204 in denatured cell lysate samples. Human and mouse reactivity was
determined by testing cell lysates with the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. Reactivity with rat samples was determined by
western blot for each of the antibodies used in the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. The biology and function of ERK1/2 has
been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master
Buffer Kit (Cat. No. 560005 or 560006).


注意事项:

This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x
concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from
prolonged exposure to light.
The Phospho ERK1/2 (T202/Y204) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL
microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat.
No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for
3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.


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