Hu Granzyme A PE Set CB9 100Tst

产品编号：BD-558904 品牌：BD-Pharmingen 原厂货号：558904
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

100Tst

运保温度:

Store undiluted at 4°C

到货周期：

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其它组分：

PE Granzyme A【子货号：51-68395X，包装：100 tests (1 ea)，，运保温度： 4°C】

PE Mouse IgG1, κ Isotype Control【子货号：51-13855X-6，包装：100 tests (1 ea)，，运保温度： 4°C】

描述：

The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule
contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a pore-forming protein, perforin, and a
group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the
granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic
pathway; granzyme B is activated immediately, while granzyme A acts hours later. Granzyme B does not induce cleavage of caspase-3, lamin
B, rho-GTPase or PARP, but does cleave DNA-PKcs and nuclear mitotic apparatus protein (NuMA). Studies involving mice which are
deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with
the granzyme A pathway acting as a backup. However, further research is needed to delineate the components of the distinct pathways.
Clone CB9 recognizes human granzyme A. Purified human granzyme A was used as immunogen. Clone MOPC-21 is a mouse IgG1 isotype
(negative) control. The MOPC-21 antibody has unknown specificity.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.