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其它组分:
注意事项:
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x
concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from
prolonged exposure to light.Recommended Assay Procedure: The BD CBA Human IgE Flex Set must be used in conjunction with a BD CBA Human Soluble Protein
Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488).
Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. The top
standard point for the BD CBA Human IgE Flex Set is 500 ng/ml and should be prepared in the following way:
For single-plex Human IgE Flex Set experiments, make the top standard dilution by mixing 20μl of the Human IgE Standard with 980μl of Assay
Diluent from the Human Soluble Protein Master Buffer Kit in a tube labeled Top Standard. Perform additional standard dilutions using the top
standard dilution by following the instructions in the Human Soluble Protein Master Buffer Kit manual.
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参考文献
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