Hu LIF PE 1F10 100Tst

• 产品编号：BD-558571      品牌：BD-Pharmingen       原厂货号：558571
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The 1F10 antibody specifically binds to human Leukemia Inhibitory Factor (LIF) also known as Human Interleukin for DA cells (HILDA),
Melanoma-derived Lipoprotein Lipase Inhibitor (MLPLI) and Hepatocyte Stimulating Factor III (HSF III). LIF is produced by multiple
sources including activated T cells and macrophages, myelomonocytic lineages, fibroblasts, liver, heart and melanoma cells. LIF regulates the
differentiation of embryonic stem cells, neural cells, osteoblasts, adipocytes, hepatocytes and kidney epithelial cells. Other activities include
terminal differentiation in leukemic cells and the stimulation of acute-phase protein synthesis in hepatocytes. Many of its biological functions
parallel those of Interleukin-6, Oncostatin M, Ciliary Neurotrophic Factor, Interleukin-11 and Cardiotrophin-1. In vivo LIF is important in
regulating the inflammatory response by tuning the balance of four systems in the body, namely the immune, the haematopoietic, the nervous
and the endocrine systems. The immunogen used to generate the 1F10 hybridoma was recombinant vaccinia virus encoding the human LIF
cytokine. The LIF cytokine had been engineered to get expressed on the membrane of the infected cell. The attachment to the membrane was
obtained with the glycosylphoshosphatidyl anchor targeting DNA sequence from the DAF molecule (CD55).

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.