Phosflow Perm Buffer III 125mL

• 产品编号：BD-558050      品牌：BD-Pharmingen       原厂货号：558050
• 产品分类：生化试剂 > 免疫分析试剂 > 流式细胞术溶液和试剂
• 应用分类：

描述：

本缓冲液用于固定的PBMC或裂解的全血的细胞透化。PBMC，可被激酶活化剂（如佛波醇酯）刺激，并且可被固定，并用缓冲液使之发生细胞膜透化作用，有利于细胞内激酶的染色。本缓冲液为FACS荧光染色设计，可与抗体一起用于磷酸化蛋白的分析（例如，对参与细胞信号传导途径的磷酸化蛋白和各种激酶等胞内蛋白的染色）。多数情况下，此类磷酸特异性抗体可同时用于细胞表面标记（例如CD分子）的染色。并非所有的细胞表面标记物都与本缓冲液兼容。

原厂资料：

BD Phosflow™ Perm Buffer III is intended to be used for the permeabilization of fixed PBMCs or lysed whole blood. PBMCs, for example, can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) followed by permeabilization of the cells with BD Phosflow™ Perm Buffer III to allow for an effective intracellular staining of kinases. Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer III can be used with antibodies for phospho-protein analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases). In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers). Not all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer III. For a current listing of compatible cell surface markers for use with BD Phosflow™ Perm Buffer III, investigators are encouraged to visit the following website: http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

注意事项：

The buffer ontains 87.68% methanol (w/w).

Hazard statements
Highly flammable liquid and vapor.
Toxic if swallowed, in contact with skin or if inhaled.
Causes damage to the central nervous system. Route of exposure: Oral.

Precautionary statements
Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
Wear protective clothing / eye protection.
Wear protective gloves.
Do not breathe mist/vapours/spray.
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.