Hu Caspase-6 Pure B93-4 100ug

• 产品编号：BD-556581      品牌：BD-Pharmingen       原厂货号：556581
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. ICE (interleukin-1ß converting enzyme) was the first
member of this family to be discovered following a search for human proteins with homology to ced-3, a cell death gene identified in C.
elegans. "Caspase" has been adopted as a root name for all family members based on the properties of these enzymes. The "c" reflects a
cysteine protease mechanism and "aspase" refers to their ability to cleave after aspartic acid residues. Caspases are synthesized as inactive
proenzymes that are processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms
consist of large (17-22 kDa) and small (10-12 kDa) subunits which 34kDa associate to form an active enzyme. Caspase-6 exists as two
isoforms, designated Mch2α (caspase-6) and Mch2ß, a proteolytically inactive isoform which lacks half of the large subunit.3 Caspase-6 is
most closely related to caspase-3, and can be proteolytically cleaved/activated by caspase-3 into large (p18) and small (p11) subunits.
Similarly, caspase-3 has been shown to be activated by caspase-6, suggesting that these proteases may generate an amplification cycle during
apoptosis. Other proteolytical targets of Western blot analysis of caspase-6. In lane 1, active caspase-6 include lamin A and a cytosolic
nuclease which translocates to the Daudi B cell lysate was probed with anti-nucleus to promote DNA fragmentation. Caspase-6 has also been
shown to cleave Fak (focal adhesion kinase), a non-receptor protein tyrosine kinase that transduces cell Clone B93-4 identifies full length
caspase-6 at survival and proliferation signals from contact sites between the cell surface and extracellular matrix. In this instance, caspase-6
cleavage inhibits the anti-apoptotic function of Fak. Therefore, the protease activity of caspase-6 plays both activating and inhibitory roles in
apoptotic pathways. Caspase-6 is observed at ~34 kDa in SDS-PAGE. The B93-4 clone recognizes human caspase-6. The antibody detects
both full length caspase-6 (34 kDa), as well as the p11 subunit of the active enzyme. A synthetic peptide sequence corresponding to amino
acids 271-285 (KKQVPCFASMLTKK) of human caspase-6 was used as immunogen.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.