Hu E2F-2 Pure TFE22 100ug

• 产品编号：BD-556525      品牌：BD-Pharmingen       原厂货号：556525
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor. E2F is now known to be a family of
related factors; E2F-1 through E2F6 have been cloned. E2F proteins form heterodimers with the related proteins, DP-1 and -2. These
complexes are regulated by their interaction with the retinoblastoma proteins (Rb, p107 and p130), which restrict E2F/DP complexes to the
cytoplasm. E2F inhibitors are phosphorylated during the cell cycle. Phosphorylation of inhibitory proteins releases the E2F/DP complex to
enter the nucleus, thus providing regulated activation of E2F responsive genes. E2F-DNA binding sites have been found in the promoter
regions of genes important for growth regulation, e.g., c-myc, N-myc, cdc2 and cyclin A and within genes which are important for DNA
synthesis. Activation of specific gene(s) may depend on the formation of distinct E2F complexes. Cotransfection studies demonstrate specific
interactions between E2F and DP proteins, i.e., E2F-1 through -5 bind to DP-1, while E2F-4 binds to DP-2. The E2Fs also display differential
binding to pRb family members. E2F gene products can promote cell cycle progression and proliferation. E2F-1, -2, and -3 are transcriptional
activators which by themselves can drive cells through S phase, and can override G1 block; E2F-4 and -5 require interaction with DP to do so.
E2F-6 is unique in that it is not regulated by pRb family members and instead acts to repress transcription activation by other E2F members.
E2F-2 migrates at a molecular weight of 60 kDa by SDS/PAGE. Clone TFE22 recognizes an epitope between amino acids 1 through 85 of
human E2F-2. It does not cross-react with other E2F proteins. The specificity of the antibody was verified by immunoprecipitation of in vitro
translated E2F-2 and by western blot analysis of cell extracts.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.