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Hu Active Caspase-3 Set 20Tst

包装：

20Tst

运保温度:

Store recombinant human active caspase-3 at -80°C

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其它组分：

Recombinant Human Active Caspase-3【子货号：51-66281V，包装：5 µg (1 ea)】

Ac-DEVD-AFC, Caspase-3 Fluorogenic Substrate【子货号：51-67201U，包装：1.0 mg (1 ea)】

Ac-DEVD-CHO, Caspase-3 Inhibitor【子货号：51-66221U，包装：1.0 mg 】

描述：

Caspases are cysteine proteases that play a central role in apoptosis. The caspase family was discovered by searching human cDNA libraries

for sequences homologous to ced-3, a C. elegans death gene that is required for normal apoptosis during development. Active caspase-3,

consisting of a 12 kDa and 17 kDa subunit derived from a 32 kDa pro-enzyme (pro- caspase-3), has been shown to cleave PARP [poly (ADP

ribose) polymerase], an enzyme that is involved in DNA repair and genomic maintenance. Proteolysis of the 116 kDa intact form of PARP

into 85 and 25 kDa subunits results in loss of normal PARP function. The cleavage site in PARP is C-terminal to Asp-216. The upstream

sequence of the PARP cleavage site, DEVD (Asp-Glu-Val-Asp), is utilized as a basis for the highly specific caspase-3 substrate Ac(N-acetyl)

-DEVD-AFC (7-amino-4-trifluoromethylcoumarin). Caspase-3 cleaves the tetrapeptide between D and AFC, thus releasing the fluorogenic

AFC which can be quantified by UV spectrofluorometry. When coupled to an aldehyde group (CHO), the DEVD peptide functions as a potent

inhibitor of caspase-3 activity and can be used to block caspase-3 mediated cleavage of Ac-DEVD-AFC. These tetrapeptide substrates can be

used to identify and quantitate caspase-3 activity in apoptotic cell lysates.

注意事项：

Avoid multiple freeze-thaws of product.

Store recombinant human active caspase-3 at -80°C prior to use or for long term storage of stock solutions.

The thawed active enzyme is generally stable at 4°C for about one week.

Active caspase-3 was expressed in E.coli and purified. When expressed in E.coli, caspase-3 will spontaneously undergo

autoprocessing to yield the subunits characteristic of the active enzyme.

Store the lyophilized Ac-DEVD-AFC substrate and the Ac-DEVD-CHO inhibitor at -20°C. Once reconstituted in DMSO, store

at -20°C for up to 2 months. Avoid multiple freeze-thaw cycles by aliquoting samples for storage.