描述:
CD56 or N-CAM (Neural cell adhesion molecule) is a member of a family of adhesion molecules including L1, contactin, TAG-1 and others,
which mediate neuronal attachment, process extension and cell-cell interaction(s) in the central nervous system (CNS). N-CAM has been
shown to play a role during the development and maintenence of synaptic connectivity of the hippocampus, as well as other organs within the
CNS. In addition to facilitating cell adhesive properties, N-CAM may play a role signal transduction pathways. N-CAM can participate in
regulation of astrocyte proliferation via a mechanism which involves the mitogen-activated protein kinase (MAPK) pathway. N-CAM
associates directly with L1 to form a complex which participates in signal pathways promoting neurite outgrowth. N-CAM exists in several
isoforms which result from alternative splicing of mRNA. These isoforms contain posttranslational modifications such as addition of polysialic
residues and carbohydrate epitopes. Embryonic N-CAM (~200-230 kD) is highly sialylated; whereas adult N-CAM isoforms (~120, 140 and
180 kD) are less so, yet are more adhesive. These modifications, along with the diversity of local distribution of the isoforms, may contribute
to distinct roles for these forms. Adult N-CAM isoforms of 120, 140 and 180 kDa are observed by SDS-PAGE.
Clone 12F11 recognizes an intracellular epitope of N-CAM and has been shown to react with human, rat, mouse, and chick N-CAM. The
antibody specifically recognizes the 140 and 180 kDa isoforms of N-CAM, but does not recognize the 120 kDa isoform. Higher molecular
weight (MW) isoforms may also be observed, particularly in samples from embryonic brain tissue.
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.