Reacts with the human ε-chain, a 20 kDa subunit of CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood
lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjuction with
pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. HIT3a antibody does not stain intracellular CD3 unlike
the other CD3 clone, UCHT1.
原厂资料:
注意事项:
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.