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Rat IL-4 Recom 5ug

 
包装: 5ug
运保温度: Store product at -80°C prior to use or for long te
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描述:

Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which can stimulate the growth, differentiation and survivability of and regulate 
the function of various cell types of hematopoietic (including B and T lymphocytes, mast cells, monocytes and macrophages) and 
non-hematopoietic (e.g., vascular endothelial cells and certain tumor cells) origin. IL-4 exerts its biological activities by binding to specific 
high affinity receptors expressed by a wide variety of cell types. The rat IL-4 gene product is a 17 -20 kD glycoprotein containing 123 amino 
acid residues. 
Formulation and Purity: Recombinant rat IL-4 is supplied as a frozen liquid comprised of 0.22 ìm sterile-filtered PBS, pH 7.2, and 
containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat IL-4 protein was 
purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the 
Beers-Lambert law. The endotoxin level is ≤0.1 ng per µg of rat IL-4, as measured in a chromogenic LAL assay.
 


注意事项:

This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Rapidly thaw and quick-spin product prior to use.
Store product at -80°C prior to use or for long term storage of stock solutions.
Avoid multiple freeze-thaws of product.
Recombinant rat IL-4 is supplied as a frozen liquid. Upon initial thawing the product should be aliquoted into polypropylene 
microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not 
less than 0.5 - 10 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro 
biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we 
recommend carrier-protein concentrations of 5 - 10 mg/ml. 
NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
 


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