This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.
注意事项：
The kit described in this product manual contain Fixation/Permeabilization solution containing 4% paraformaldehyde, and either BD GolgiStop™ protein transport inhibitor containing 99.9% ethanol, or BD GolgiPlug™ protein transport inhibitor containing 99.9% dimethylsulfoxide and 0.1% brefeldin A.
The following warnings and precautions apply to the aforementioned chemicals.
R 36/38 Irritating to eyes and skin S3 Keep in a cool place
S26 In case of contact with eyes, rinse immediately with water and seek medical advise
S28 After contact with skin, wash immediately with plenty of water
R40 Limited evidence of a carcinogenic effect
S36/37 Wear suitable protective clothing and gloves
* Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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