Ms IL-13 Recom 5ug

产品编号：BD-554599 品牌：BD-Pharmingen 原厂货号：554599
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 小鼠
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

5ug

运保温度:

Store product at -80°C prior to use or for long te

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描述：

Interleukin-13 (IL-13) is a cytokine produced by activated T lymphocytes which regulates the function of mouse macrophages and shares some biological functions with IL-4. Administration of mouse IL-13 to cultured mouse macrophages induces morphological changes (flattening, and development of processes), leads to increases in class II MHC antigen expression, and inhibits the synthesis of several proinflammatory cytokines (including IL-1β, IL-6, TNF, and IL-12). In contrast to human IL-13, mouse IL-13 does not appear to have activity for mouse B cells. Recombinant mouse IL-13 (mIL-13) has multiple glycosylated forms ranging in molecular weights from 11.3 to 15 kD, as detected by SDS-PAGE analysis.

Formulation and Purity:

Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-13 is ≥95% pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law. The endotoxin level is ≤0.1 ng per µg of mouse IL-13, as measured in a chromogenic LAL assay.

Preparation and Storage

Store product at -80°C prior to use or for long term storage of stock solutions.

Avoid multiple freeze-thaws of product.

Rapidly thaw and quick-spin product prior to use.

Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
Avoid multiple freeze-thaws of product.
Rapidly thaw and quick-spin product prior to use.
Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.
Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such
as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend
carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5
- 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should
not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an
appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or
possible blocking activity.