Hu E2F-1 Pure KH95/E2F 100ug

• 产品编号：BD-554213      品牌：BD-Pharmingen       原厂货号：554213
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter.
E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and
cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA
polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes
with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety
of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent
trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This
dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an
active “E2F” transcription factor. E2F-1 migrates at a reduced molecular weight of ~60 kDa. Clone KH95/E2F (also referred to as KH95)
recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially
expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture
supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.