Hu Cyclins D1/D2/D3 Pure G124-259 100ug

• 产品编号：BD-554203      品牌：BD-Pharmingen       原厂货号：554203
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclins and cyclin-dependent kinases (cdks) are evolutionary conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins
(regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks
complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These
complexes are regulated by activating and inhibitory phosphorylation events as well as by interactions with small proteins that bind to cyclins,
cdks, or cyclin-cdk complexes, e.g., p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones,
oncogenes (c-src, c-abl, SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II
and others. It is thought that D-type cyclins are involved in regulating in the passage of mammalian cells through G1. The reduced molecular
weights of D-type cyclins are as follows: cyclins are D1 (36 kDa), cyclin D2 (35 kDa) and cyclin D3 [31 and 34 kDa (doublet)]. G124-259
recognizes human cyclins D1 (36 kDa), D2 (35 kDa), and D3 (31 and 33 kDa). Recombinant full-length human cyclin D1, expressed in the
Baculovirus Expression System (BEVS), was used as immunogen. Hybridomas were selected by ELISA and western blot reactivity. G124-259
was selected, as it reacted with D1, D2, and D3, apparently recognizing a common epitope among these three cyclin D proteins.
D-type cyclins are differentially expressed in distinct cell types. This differential expression appears to exist even among cell types which
generally have high levels of proliferative proteins. For example, whereas cyclin D1 was readily detected in a human glioblastoma cell line
(U118 MG), it was undetected in transformed primary human embryonic kidney cells (293). Cell types which have been documented to
express high levels of a given D-type cyclin are suggested as positive controls. WI-38 human diploid fibroblasts (ATCC CCL 75) and U-118
(ATCC HTB 15) are suggested as positive controls for detecteing cyclin D1 and D3. Primary human peripheral blood T lymphocytes
stimulated with phytohemagglutin in (PHA) and Raji human Burkitt lymphoma cells (ATCC CCL 86) are suggested as positive controls for
detecting cyclin D2.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.