Rat Ig HRP Poly 1271 1mL

• 产品编号：BD-554017      品牌：BD-Pharmingen       原厂货号：554017
• 产品分类：抗体 > 二抗 > 酶标记
• 应用分类：

原厂资料：

HRP Goat Anti-Rat Ig is a polyclonal secondary antibody used for the detection of rat Ig. This antibody is tested by ELISA. Investigators are
highly encouraged to titrate the reagent for specific applications (1:1000 dilution is the suggested starting dilution). It is reactive in ELISA
with rat IgG, IgM, and IgA; a weaker signal may be detected on the rat IgG2b and IgM isotypes. It has some cross-reactivity with mouse IgG
and no cross-reactivity with human Ig. For immunohistochemical staining (IHC) of acetone-fixed frozen and paraffin-embedded sections, the
use of biotinylated goat anti-rat Ig (Cat. No. 559286) is recommended. However, when using rat IgG2b primary antibodies, biotinylated mouse
anti-rat IgG2b mAb G15-337 (Cat. No. 550327) is recommended as second step in IHC. When using rat IgM primary antibodies in IHC,
biotinylated anti-rat and -hamster Ig κ light chain mAb RG7/7.6 (Cat. No. 550336) or biotinylated anti-rat IgM mAb G53-238 (Cat. No.
550330) as second step is recommended.

注意事项：

Store undiluted at -20°C.
Avoid multiple freeze-thaws of product.
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
The antibody solution was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to
minimize cross-reactivity with mouse, human, cow, and horse serum proteins (multiple adsorption).