The IM7 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The standard form of
CD44, lacking variable exons and referred to as CD44H or CD44s, is widely expressed on hematopoietic and non-hematopoietic cells. CD44
isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1
alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A,
AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have few CD44H+ T cells. CD44 is a cell adhesion receptor, and
its principal ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding
to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1). Bone marrow- and
thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases
upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells; memory cells can be recognized by their CD44[hi] phenotype. The IM7
mAb inhibits established collagen-induced arthritis in DBA/1 mice. Moreover, it prevents CNS inflammation and clinical symptoms of
experimental autoimmune encephalomyelitis. In contrast, the same antibody exacerbates experimental autoimmune thyroiditis in CBA/J mice.
The IM7 mAb recognizes a different epitope from that recognized by mAb KM114 (Cat. No. 558739), and the antibody pair can be used in
ELISA to detect soluble CD44. It has been observed that IM7 antibody cross-reacts with human, dog, cat, horse, cow, and pig leukocytes.
Anti-human CD44, clone G44-26 (Cat. No. 555476), and IM7 antibody compete for binding to human peripheral blood lymphocytes.
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Store undiluted at 4°C.
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